404 error with Umbraco directory urls

I'd like to enabled Umbraco directory urls for an Umbraco project I'm working on. I've set the umbracoUseDirectoryUrls config setting to true. Unfortunately I'm now getting 404 errors on all pages without extension (with extension they're still working). Below are the configuration details:

• IIS 7.5 on Windows 7 x64
• Umbraco version 4.0.1
• Appl开发者_如何学Cication pool is running in .Net 2.0 classic mode
• I've added a wildcart script mapping in IIS to aspnet_isapi.dll before the StaticFile handler

The 404 error always originates from the StaticFile handler. I don't understand why it doesn't go through the isapi handler.

Unfortunately I will not be able to upgrade Umbraco, change to integrated mode, or change to .NET 4 at this moment.

tried "republish entire site" after setting the usedirectoryurls to true ? You can find it when right clicking the content node in the content section

It probably had something to do with some other configuration setting. After I started with an original Umbraco web.config I was able to switch to integrated mode, which made everything a lot easier.