IIS application with virtual directories running code in ASP.NET

If I have an ASP.NET application in IIS with virtual directories, can aspx pages in the virtual directories access DLLs within that app开发者_JAVA技巧lication as well?

WEBSITE  
 -App___Data  
 -App_Themes  
 -Bin  
   -MyDLL.dll  
 -Images  
 -MyVirtualDir  
   -MyAspPageThatNeedsToAccessMyDll.aspx  

Is this possible?

Yes, assuming that all the folders are running under the same application. If the "MyVirtualDir" was its own application, then I don't think it'll work.