mod_rewrite: url rewriting plus subdomain (wildcard) rewrite at the same time

I have two rules in my .htaccess file for url rewriting:

1. for subdomain rewriting: xxx.domain.com is internally redirected to file.php?item=xxx

   RewriteCond %{HTTP_HOST} !^www\.domain\.com$ [NC]
   
   RewriteCond %{HTTP_HOST} ^(www\.)?([^\.]+)\.domain\.com$ [NC]
   
   RewriteRule ^$ /file.php?item=%2 [QSA,nc]
   

2. ordinary rewriting:

   RewriteRule ^([A-Za-z0-9_)(:!-',]+)/?$ file.php?item=$1 [L] 
   

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What i need to achieve is writing a third rule that will combine these two rules without being in conflict with them. Namely, below (or above) this lines i need to have something like that:

RewriteCond %{HTTP_HOST} ^(www\.)?([^\.]+)\.domain\.com/([A-Za-z0-9_)(:!-',]+)$ [NC]

RewriteRule ^$ /anotherfile.php?item1=%2&item2=$1 [QSA,nc]

so that http://xxx.domain.com/yyy will be redirected to anotherfile.php?item1=xxx&item2=yyy

any ideas that will work, or what is the correct way of it?

Try this rule:

RewriteCond %{HTTP_HOST} !^www\.example\.com$ [NC]
RewriteCond %{HTTP_HOST} ^(www\.)?([^.]+)\.example\.com$ [NC]
RewriteRule ^([A-Za-z0-9_)(:!-',]+)/?$ /file.php?item1=%2&item2=$1 [QSA]

It uses conditions of the first rule and URL path pattern of the second.