Is it possible to block end users from using Javascript to trigger events? [closed]

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As an example, let's assume I want to prevent users from using auto-clickers. Is it possible, and are there accepted best practices when doing this?

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Nope, it is not possible. Someone can always turn off JavaScript, modify it, run their own JS-code, etc, etc. JavaScript should never ever be used for security reasons.

However, for AJAX-requests you could implement a minimum interval server-side (like on Stack Overflow).